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Transgenomic wave™ (dhplc) dna fragment analysis system
Wave™ (Dhplc) Dna Fragment Analysis System, supplied by Transgenomic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dhplc+analysis/wave+dhplc+system/pmc01808054-188-18-24
Average 90 stars, based on 1 article reviews
wave™ (dhplc) dna fragment analysis system - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

High Performance Liquid Chromatography:

Article Title: The C20orf133 gene is disrupted in a patient with Kabuki syndrome
Article Snippet: The C20orf133 ( {"type":"entrez-nucleotide","attrs":{"text":"AK131348","term_id":"47077161","term_text":"AK131348"}} AK131348 ) and the FLRT3 (a nested gene located within intron 3 of C20orf133 ) genes were amplified using primer sets for each exon (supplementary table 2; available online at http://jmg.bmj.com/supplemental ), and PCR products were sequenced in both directions, then analysed (ABI3130; Applied Biosystems and Ensembl database, release 42). .. Denaturating high‐performance liquid chromatography (DHPLC) analysis was performed according to the manufacturer's instructions (Transgenomic Wave system; Transgenomic, Cheshire, UK) on genomic DNA of C20orf133 exon 14, using the primers 5′‐TGC‐ATA‐TCA‐CAT‐TTC‐TTT‐TAT‐TTT‐TCA‐3′ and 5′‐CCA‐CGC‐ACA‐CAC‐ACA‐GGT‐AT‐3′. .. A 10 μl aliquot of approximately 10 ng/μl crude PCR product was loaded onto a chromatography column (DNASep; Transgenomic).

Polymerase Chain Reaction:

Article Title: Complete screening of 50 patients with CHARGE syndrome for anomalies in the CHD7 gene using a denaturing high-performance liquid chromatography-based protocol: new guidelines and a proposal for routine diagnosis.
Article Snippet: .. PCR amplicons obtained from human genomic DNA were subjected to DHPLC analysis (Transgenomic, Glasgow, UK). ..

Sequencing:

Article Title: Genotype-phenotype analysis and natural history of left ventricular hypertrophy in LEOPARD syndrome.
Article Snippet: Genotype–Phenotype Analysis and Natural History of Left Ventricular Hypertrophy in LEOPARD Syndrome Giuseppe Limongelli,* Anna Sarkozy, Giuseppe Pacileo, Paolo Calabrò, Maria Cristina Digilio, Valeria Maddaloni, Giulia Gagliardi, Giovanni Di Salvo, Maria Iacomino, Bruno Marino, Bruno Dallapiccola, and Raffaele Calabrò Monaldi Hospital, Second University of Naples, Naples, Italy IRCCS-CSS, San Giovanni Rotondo and CSS-Mendel Institute, Rome, Italy Department of Experimental Medicine and Pathology, University ‘‘La Sapienza’’, Rome, Italy Medical Genetics, Bambin Gesu’ Hospital, Rome, Italy Cardiology, Bambin Gesu’ Hospital, Rome, Italy Department of Pediatrics, ‘‘La Sapienza’’ University, Rome, Italy

Article Title: A founder MLH1 mutation in Lynch syndrome families from Piedmont, Italy, is associated with an increased risk of pancreatic tumours and diverse immunohistochemical patterns.
Article Snippet: .. DHPLC, MLPA and sequencing Screening for point mutations was performed by DHPLC analysis (Transgenomic, Inc., Omaha, NE, USA). .. PCR products with heteroduplex profiles were sequenced on an ABI 3100 Avant sequencer (Applied Biosystems, Foster City, CA, USA).

DNA Sequencing:

Article Title: Genotype-phenotype analysis and natural history of left ventricular hypertrophy in LEOPARD syndrome.
Article Snippet: Genotype–Phenotype Analysis and Natural History of Left Ventricular Hypertrophy in LEOPARD Syndrome Giuseppe Limongelli,* Anna Sarkozy, Giuseppe Pacileo, Paolo Calabrò, Maria Cristina Digilio, Valeria Maddaloni, Giulia Gagliardi, Giovanni Di Salvo, Maria Iacomino, Bruno Marino, Bruno Dallapiccola, and Raffaele Calabrò Monaldi Hospital, Second University of Naples, Naples, Italy IRCCS-CSS, San Giovanni Rotondo and CSS-Mendel Institute, Rome, Italy Department of Experimental Medicine and Pathology, University ‘‘La Sapienza’’, Rome, Italy Medical Genetics, Bambin Gesu’ Hospital, Rome, Italy Cardiology, Bambin Gesu’ Hospital, Rome, Italy Department of Pediatrics, ‘‘La Sapienza’’ University, Rome, Italy

Article Title: Molecular analysis of PRKAG2, LAMP2, and NKX2-5 genes in a cohort of 125 patients with accessory atrioventricular connection.
Article Snippet: Molecular Analysis of PRKAG2, LAMP2, and NKX2-5 Genes in a Cohort of 125 Patients With Accessory Atrioventricular Connection Giorgia Esposito, Giorgia Grutter, Fabrizio Drago, Mauro W. Costa, Antonella De Santis, Giovanna Bosco, Bruno Marino, Emanuele Bellacchio, Francesca Lepri, Richard P. Harvey, Anna Sarkozy,* and Bruno Dallapiccola IRCCS-Casa Sollievo della Sofferenza, San Giovanni Rotondo, Rome, Italy Casa Sollievo della Sofferenza-Mendel Institute, Rome, Italy Department of Experimental Medicine and Pathology, University ‘‘La Sapienza’’, Rome, Italy Department of Pediatric Cardiology, Bambino Gesu?. Hospital, IRCCS, Rome, Italy Victor Chang Cardiac Research Institute, Darlinghurst, Sydney, Australia Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil Section of Pediatric Cardiology, Department of Pediatrics, University ‘‘La Sapienza’’, Rome, Italy

Mutagenesis:

Article Title: Molecular analysis of PRKAG2, LAMP2, and NKX2-5 genes in a cohort of 125 patients with accessory atrioventricular connection.
Article Snippet: Molecular Analysis of PRKAG2, LAMP2, and NKX2-5 Genes in a Cohort of 125 Patients With Accessory Atrioventricular Connection Giorgia Esposito, Giorgia Grutter, Fabrizio Drago, Mauro W. Costa, Antonella De Santis, Giovanna Bosco, Bruno Marino, Emanuele Bellacchio, Francesca Lepri, Richard P. Harvey, Anna Sarkozy,* and Bruno Dallapiccola IRCCS-Casa Sollievo della Sofferenza, San Giovanni Rotondo, Rome, Italy Casa Sollievo della Sofferenza-Mendel Institute, Rome, Italy Department of Experimental Medicine and Pathology, University ‘‘La Sapienza’’, Rome, Italy Department of Pediatric Cardiology, Bambino Gesu?. Hospital, IRCCS, Rome, Italy Victor Chang Cardiac Research Institute, Darlinghurst, Sydney, Australia Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil Section of Pediatric Cardiology, Department of Pediatrics, University ‘‘La Sapienza’’, Rome, Italy

Multiplex Ligation-dependent Probe Amplification:

Article Title: A founder MLH1 mutation in Lynch syndrome families from Piedmont, Italy, is associated with an increased risk of pancreatic tumours and diverse immunohistochemical patterns.
Article Snippet: .. DHPLC, MLPA and sequencing Screening for point mutations was performed by DHPLC analysis (Transgenomic, Inc., Omaha, NE, USA). .. PCR products with heteroduplex profiles were sequenced on an ABI 3100 Avant sequencer (Applied Biosystems, Foster City, CA, USA).



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